Molecular Biology & Metabolomics
Metabolomics is the study of small molecules counting metabolic intermediates (aminoacids, nucleotides, sugars etc.) that exists between cells. The metabolome dispense a sensitive indicator of the physiological status of a cell and has potential uses in monitoring diseases and its management. Since many proteins are enzymes acting upon small molecule , it follows that alterations to the proteome arising from environmental change, disease etc., will be reflected in the metabolome, the entire set of small molecules - aminoacids, nucleotides, sugars etc. and all the intermediates that exist within a cell during their synthesis and degradation. Metabolomics or metabolic profiling is the quantitative analysis of all such cellular metabolites at any one time under defined conditions. Because of the dissimilar chemical nature of minor cellular metabolites a variety of methods is required to measure these including gas chromatography, high performance liquid chromatography and capillary electrophoresis coupled to NMR and mass spectrometry. The yield from these methods can generate fingerprint of groups of related compounds.